Journal: Nanomaterials

Article Title: The Influence of Polysaccharide Coating on the Physicochemical Parameters and Cytotoxicity of Silica Nanoparticles for Hydrophilic Biomolecules Delivery

doi: 10.3390/nano9081081

Figure Lengend Snippet: Effect of uncoated and coated SiNP on viability of Caco-2 cells after 24 or 48 h exposure to 50, 100, 200 and 500 µg/mL of SiNP ( A ), SiNP-CH ( B ) and SiNP-CH ( C ). Cell viability is expressed as % of control (n = 8) and data are presented as means ± SD. Statistical difference between control group and formulations is reported as * p ˂ 0.05.

Article Snippet: HepG2 (Human hepatocellular carcinoma cell line; ATCC ® Number: HB-8065TM) was a gift from Professor Carlos Palmeira (CNC-UC, Coimbra, Portugal) and Caco-2 (Human colon adenocarcinoma cell line) was purchased from Cell Lines Service (CLS, Eppelheim, Germany).

Techniques:

Journal: Cell Death Discovery

Article Title: Targeting oxeiptosis-mediated tumor suppression: a novel approach to treat colorectal cancers by sanguinarine

doi: 10.1038/s41420-023-01376-3

Figure Lengend Snippet: A CRC cells were treated with SNG in the presence or absence of Cat. Cell viability was assessed by MTT assay. Data shown are means ± SD ( n = 3) (*** p < 0.001). CRC cells were treated with SNG in the presence or absence of Sod-Py. B ROS assay was performed by using DCFH-DA and C Cell viability using MTT assay was carried out. Data shown are means ± SD ( n = 3) (** p < 0.01 and *** p < 0.001) D HT-29 cells were treated with 10 µM MitoPY1 for 1 h at 37 °C. The medium was replaced with fresh growth media containing Hoechst 33342 dye (1.5 µl; 10 mg/ml stock solution). Following the treatment with SNG in the presence or absence of Sod-Py for 30 min, the fluorescence of Hoechst (blue), and MitoPY1 (green) was captured by fluorescent microscopy. H 2 O 2 (1 mM) was used as a positive control. Scale bar: 20 µm. E HT-29 and F CaCo-2 cells were treated with H 2 O 2 (500 µM) in the presence or absence of indicated inhibitors. Following the treatment, cell viability was performed by MTT assay. Data shown are means ± SD ( n = 3) (*** p < 0.001, ns = no significance). G Representative TEM images of HT-29 cells treated with SNG in the presence or absence of NAC for 16 h. The yellow arrowheads indicate plasma membrane and the white arrowhead indicates nucleus (PM, plasma membrane; N, nucleus). Scale bar: 2 µm.

Article Snippet: Human CRC cell lines (CaCo-2, HCT-116, and HT-115), were procured from The European Collection of Authenticated Cell Cultures (ECACC).

Techniques: MTT Assay, ROS Assay, Fluorescence, Microscopy, Positive Control, Clinical Proteomics, Membrane

Journal: Cell Death Discovery

Article Title: Targeting oxeiptosis-mediated tumor suppression: a novel approach to treat colorectal cancers by sanguinarine

doi: 10.1038/s41420-023-01376-3

Figure Lengend Snippet: CRC cells were stably transfected with PGAM5-shRNAs. After transfection, cells were treated with SNG (4 μM) for 16 h. Following the treatment, A Western blot analysis of indicated proteins was performed. The signal intensities of western blot bands were normalized to AIFM1 or actin of each group, and fold changes were plotted in a histogram from three independent experiments. Significant difference, ** p < 0.01 and *** p < 0.001. B CRC cells were stably transfected with PGAM5-shRNAs. After transfection, cells were treated with the indicated concentration of SNG for 16 h and then cell viability was measured by MTT assay. Data shown are mean ± SD ( n = 3) (* p < 0.05, ** p < 0.01 and *** p < 0.001) and C crystal violet staining was performed. Scale bar: 10 µm. Indicated cells were stably transfected with AIFM1-shRNA, after transfection, cells were treated with the indicated concentrations of SNG (HT-29 for 16 h and CaCo-2 for 6 h), respectively. Following the treatment, D and E cell viability was measured by MTT assay. Data shown are mean ± SD ( n = 3) (* p < 0.05, ** p < 0.01 and *** p < 0.001), and F crystal violet staining was performed. Scale bar: 10 µm. G HT-29 cells were treated with SNG in the presence or absence of NAC for 16 h. Whole cell lysates of treated as well as untreated cells were subjected to immunoprecipitation with the respective antibodies as indicated, followed by detection precipitates (top) and input lysates (bottom) with the appropriate antibodies by Western blotting. HT-29 cells co-treated with H 2 O 2 (250 μM) were exposed to SNG (2 μM) in the presence or absence of NAC. H Western blot analysis of indicated proteins was performed. The signal intensities of western blot bands were normalized to AIFM1 of each group, and fold changes were plotted in a histogram from three independent experiments. Significant difference, * p < 0.05, *** p < 0.001 and ns = no significance, I Cell viability was measured by using MTT assay. Data shown are mean ± SD ( n = 3) (* p < 0.05, *** p < 0.001).

Article Snippet: Human CRC cell lines (CaCo-2, HCT-116, and HT-115), were procured from The European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Stable Transfection, Transfection, Western Blot, Concentration Assay, MTT Assay, Staining, shRNA, Immunoprecipitation